PENGARUH BENZYL AMINO PURIN DAN ARANG AKTIF TERHADAP TINGGI TUNAS DAN JUMLAH TUNAS KRISAN (Chrysanthemum daisy L.) SECARA IN VITRO

Nurmayulis ., Susiyanti ., Khafas Bastian

Abstract


ABSTRACT

This research was about the growth of in vitro Chrysanthemum daisy L.) on medium which used Benzyl Amino Purine (BAP) and activated  charcoal. Research aim to determine the growth of Chrysanthemum plant on different concentrations of BAP and active charcoal into medium. This reseach was done in culture laboratory, in Association of Plant Forest Seed and Plantation Banten Province.  This research used factorial experiment which arranged in Randomized  Complete Design. The treathment consist of two factor. The first factor was BAP concentrate which were consist of five level include 0 ppm concentrate; 0.5 ppm; 1.0 ppm; 1.5 ppm; and 2.0 ppm. Second factor was activated charcoal dossage which consist four level included dossage 0 g L-1; 1 g L-1; 2 g L-1; and 3 g L-1. Based on the result of research showed that interaction in BAP and active charcoal influenced hight bud, but did not influence the number of shoots.  The best high of shoots on the age 1-5 WAP (Week After Plants) which given BAP and activated charcoal in vitro medium consist act combination 1-5 WAP A0B1 (0.5 ppm BAP without active charcoal). Singular factor BAP and active charcoal showed that act without BAP and activated charcoal produced the best at 5 WAP.

Keywords: Chrysanthemum  plant, BAP, and Activated charcoal


Keywords


Chrysanthemum plant, BAP, and Activated charcoal.

Full Text:

PDF

References


DAFTAR PUSTAKA

Abidin, Z. 1990. Dasar-dasar tentang zat pengatur tumbuh. Angkasa. Bandung.

Anonimous. 2007. Kultur jaringan. http/www.Agrobiogen.co.id. Diakses tanggal 16 September 2008.

Anonimous. 2009. Krisan. http://www.ibujempol.com/bunga-krisan/. Diakses tanggal 22 Januari 2009.

Anonimous. 2009. Krisan.http://www.ristek.go.id/krisan/. Diakses tanggal 22 Januari 2009.

Arie, C. 2004. Pengaruh BAP dan IAA terhadap perbanyakan tanaman kubis hias (Brassica oleraceae sub spesies acephala) secara in vitro. Institut Pertanian Bogor. Bogor.

Daisy, P.S., dan A. Wijayani. 1994. Teknik kultur jaringan, pengendalian dan petunjuk perbanyakan tanaman secara vegetatif modern. Kanisius. Yogyakarta.

Farid, M. 2003. Perbanyakan tebu (Saccharum officinarum L.) secara in vitro pada berbagai konsentrasi IBA dan BAP. Jurusan Budidaya Pertanian. Unhas.

Gunawan, L.W. 1987. Teknik kultur jaringan. Pusat Antar Universitas Bioteknologi. Institut Pertanian Bogor. Bogor.

Gunawan, L.W. 1992. Teknik Kultur Jaringan. Pusat Antar Universitas Bioteknologi. Institut Pertanian Bogor. Bogor.

Hartmann, H.T., dan D.E. Kester. 1983. Plant propagation, principles and practise 4thEd. Prentice Hall Inc. Englewood Cliffs. New Jersey.

Maryani, Y., dan Zamroni. 2005. Penggandaan tunas krisan melalui kultur

jaringan multiplication of crisan bud through tissue culture. Balai Benih Induk. Magelang. Jawa tengah.

Novita, V. 2006. Kultur in vitro jarak pagar (Jatropha curcas L.) melalui penggunaan pemadat hidrogel (aquasorb TM) dan gula trehalosa (treha TM). Institut Pertanian Bogor. Bogor.

Pararaja, A. 2008. Karbon aktif. http//www.google.co.id/arang aktif/. Diakses tanggal 16 September 2008.

Rukmana, R., dan A. E. Mulyana. 1997. Krisan. Kanisius. Yogyakarta.

Santoso, U., dan F. Nursandi. 2003. Kultur jaringan tanaman. UMM Press. Malang.

Sofia, D. 2007. Pengaruh berbagai konsentrasi BAP dan cycocel terhadap pertumbuhan embrio kedelai (Glycine max L. Merr) secara in vitro. Fakultas Pertanian, Universitas Sumatera Utara. Medan.

Susiyanti. 2008. Materi pelatihan kultur jaringan. Fakultas Pertanian, Universitas Sultan Ageng Tirtayasa Serang. Serang.

Wattimena, G.A. 1992. Biotekhnologi tanaman. Laboratorium Kultur Jaringan. Depdikbud Dirjen DIKTI PAU Biotekhnologi IPB. Bogor.

Widiastuty, D., dan Marwoto, B. 2007. Pengaruh berbagai sumber arang dalam media kultur in vitro terhadap pertumbuhan plantlet oncidium. Balai Penelitian Tanaman Hias. Cianjur.




DOI: http://dx.doi.org/10.33512/j.agrtek.v2i2.646

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Jurnal Agroekoteknologi

 

INDEXED BY:

      

 

   crossref-logo-landscape-200 


View My Stats Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.