Polylactic acid Synthesis via Direct Polycondensation Method Using Candida rugosa Lipase Catalyst
Abstract
Polylactic acid (PLA) is a biodegradable, biocompatible polymer and is produced from renewable natural resources. PLA synthesis through a polycondensation mechanism generally uses a metal catalyst, such as Zn and Sn oxides. The disadvantages of using metal catalysts are the contamination of products produced by the metals used so that unsafe products are used for biomedical applications and food packaging. The purpose of this research is to synthesize the safe PLA used for food packaging and biomedical applications. Polycondensation is done by pretreatment of lactic acid at 120oC for 1 hour. Diluted lactic acid is mixed with Candida rugosa lipise catalyst with a certain concentration (1, 2, 3 and 4% w / w). The mixture was heated at certain temperature (60; 80; 100 and 120oC) and vacuum pressure of 0.1 bar for 72 hours. The highest viscosity and density of PLA in this study was produced at 100oC and using 3% Candida rugosa lipase. The highest viscosity and density of PLA were 2443,9 CSt and 1231,9 mg / l respectively. Candida rugosa lipase concentration does not affect PLA yield at constant polycondensation temperature. PLA yield is affected by temperature. PLA yields at 60, 80, 100 and 120 ° C are 97.98; 97,65; 96.78; 96.13% respectively. The molecule weight of PLA at temperature 100oC for 1436-1482. Lipase concentration does not affect the molecular weight of the PLA.
Full Text:
PDFReferences
Achmad, Feerzet, Yamane, Kenji, Quan, Shi, & Kokugan, Takao. (2009).
Synthesis of polylactic acid by direct polycondensation under
vacuum without catalysts, solvents and initiators. Chemical
Engineering Journal, 151(1), 342-350.
Gao, Chao, Ma, Cuiqing, & Xu, Ping. (2011). Biotechnological routes
based on lactic acid production from biomass. Biotechnology
advances, 29(6), 930-939
García-Arrazola, Roeb, López-Guerrero, David A, Gimeno, Miquel, &
Bárzana, Eduardo. (2009). Lipase-catalyzed synthesis of poly-llactide using supercritical carbon dioxide. The Journal of
Supercritical Fluids, 51(2), 197-201.
Groot, W., et al., (2010). Production and Purification of Lactic Acid and Lactide, in Poly(Lactic Acid), John Wiley & Sons, Inc. p. 1-18.
Guzmán-Lagunes, Fernando, López-Luna, Alberto, Gimeno, Miquel, & Bárzana, Eduardo. (2012). Enzymatic Synthesis of Poly-< i> L-
lactide in Supercritical R-134a. The Journal of Supercritical Fluids.
Hassouna, F., et al.,(2011). New approach on the development of plasticized polylactide (PLA): Grafting of poly(ethylene glycol) (PEG) via reactive extrusion. European Polymer Journal. 47(11): p. 2134-2144.
Heise A, Palmans ARA. (2010). Hydrolases in polymer chemistry:chemoenzymatic approaches to polymeric materials. Adv Polym Sci;237: 79-113.
Kiran, K.R. and S. Divakar, (2003). Lipase-catalysed polymerization of lactic acid and its film forming properties. World Journal of Microbiology and Biotechnology. 19(8): p. 859-865.
Kobayashi. (2009). Recent developments in lipase-catalyzed synthesis of polyesters. Macromol Rapid Commun;30:237-66.
Lasprilla, A.J., et al., (2012). Poly-lactic acid synthesis for application in biomedical devices—A review. Biotechnology advances. 30(1): p.
-328.
Lassalle, V. L. & Ferreire M. L. (2008). Lipase-catalyzed synthesis of polylactic acid; An overview of the experimental aspects. Journal of Chemical technology and Biotechnology, Vol.83, No.11, pp. 1493-1502
Lopes, M.S. and A. Jardini, (2012). Poly (lactic acid) production for tissue engineering applications. Procedia Engineering. 42: p. 1530-1542.
Madhavan Nampoothiri, K., N.R. Nair, and R.P. John, (2010). An overview of the recent developments in polylactide (PLA) research. Bioresource technology. 101(22): p. 8493-8501.
Maharana, T, Mohanty, B, & Negi, YS. (2009). Melt–solid polycondensation of lactic acid and its biodegradability. Progress in Polymer Science, 34(1), 99-124.
Namekawa, Shuhei, Suda, Sakae, Uyama, Hiroshi, & Kobayashi, Shiro. (1999). Lipase-catalyzed ring-opening polymerization of lactones to polyesters and its mechanistic aspects. International Journal of Biological Macromolecules, 25(1), 145-151.
Rasal, R. M.: Janorkar, A. V. & Hirt, D.E, (2010). Poly(lactic acid) modifications. Progress in Polymer Science, Vol.35, No.3, pp.338-356.
Sheth,M. et al., (1997). Biodagradable polymer blends of poly(lactic
acid) and poly(ethylene glycol). Journal of Applied Polymer Science, Vol.66, No.8, pp. 1495-1505.
Södergård, A. and M. Stolt, (2010). Industrial Production of High Molecular Weight Poly(Lactic Acid), in Poly(Lactic Acid), John Wiley & Sons, Inc. p. 27-41.
Taguchi, Seiichi, Yamada, Miwa, Matsumoto, Ken'ichiro, Tajima, Kenji, Satoh, Yasuharu, Munekata, Masanobu, Kambe, Hiromi. (2008). A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme. Proceedings of the National Academy of Sciences, 105(45), 17323-17327.
Tsukegi.T, Motoyama.T, Shirai.Y, Nishida.H, Endo.T.(2007). Racemization behavior of L,L-lactide during heating. Polymer Degradation and Stability, 92, 552-559
Xiao, Lin, Wang, Bo, Yang, Guang, & Gauthier, Mario. Poly (Lactic Acid)-Based Biomaterials: Synthesis, Modification and Applications. Biomedical Science, Engineering and Technology.
Yang, Yan, Yu, Yang, Zhang, Yuanxin, Liu, Chengbai, Shi, Wei, & Li, Quanshun. (2011). Lipase/esterase-catalyzed ring-opening polymerization: A green polyester synthesis technique. Process Biochemistry, 46(10), 1900-1908.
Yoo, Dong-Keun, & Kim, Dukjoon. (2009). Production of optically pure poly (lactic acid) from lactic acid. Polymer Bulletin, 63(5), 637-
DOI: http://dx.doi.org/10.62870/wcej.v1i5.2621
Refbacks
- There are currently no refbacks.
Copyright (c) 2017 World Chemical Engineering Journal
WCEJ (e-ISSN: 2443-2261) is published by Chemical Engineering Department, Universitas Sultan Ageng Tirtayasa (UNTIRTA).
This Journal has been indexed by:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.