INIASI TUNAS SECARA KULTUR JARINGAN PADA STEVIA (Stevia rebaudiana Bertoni) DENGAN KOSENTRASI INDOLE BUTYRIC ACID (IBA) AND BENZYL AMINO PURINE (BAP) YANG BERBEDA

Anisa Hadiyana, Mohamad Ana Syabana, Susiyanti .

Abstract


This research aims to know the initiation of bud stevia (Stevia rebaudiana Bertoni) in several concentrations of Indole Butyric Acid (IBA) and Benzyl Amino purine (BAP) are in in vitro. This research was conducted from August to October 2014 in engineering laboratory, Faculty of agriculture, University of Sultan Ageng Tirtayasa, Serang, Banten. This study used a Random group of Design with two factors. The first factor was the concentration of IBA 3 adequate for the applicable Io (0 mg/l), I1 (0.5 mg/l), I2 (1 mg/l) and the second factor is the concentration of BAP with 3 levels, apply B0 (0 mg/l), B1 (0.5 mg/l), B2 (1 mg/l). So, there are a total of 9 combination treatments repeated 3 times so that there are 27 units of the experiment. The results showed that treatment without IBA and BAP (I0B0) gave the best result in parameter first appearance of shoots (4 days after planting) and shoot length (0,85 cm). While, the treatment I2B2 (1 mg/l IBA and BAP) gave the best result in parameter leaves number (22 leaves).


Keywords: initiation, stevia, growing regulatory substances, in vitro


Keywords


tanaman

Full Text:

PDF

References


Abbas, B. 2011. Prinsip Dasar Teknik Kultur Jaringan. Alfabeta, Bandung.

Ahmed, E. E., GY.D. Bisztray and I. Velich. 2002. Plant regeneration from seedling explants of common bean (Phaseolus vulgaris L.). Proceedings of the 7th Hungarian Congress on Plant Physiology. Szent Istvan University of Budapest. Budapest, Hungary. 115 - 123.

Hassanen, S. A. and Khalil, R. M. A. 2013. Biotechnological Studies for Improving of Stevia (Stevia

rebaudiana Bertoni) in vitro Plantlets. Middle-East Journal of Scientific Research 14 (1): 93-1006.

Prihatmani, D dan N. A. Mattjik, 2004. Penggunaan Zat Pengatur Tumbuh NAA (Naphtalene Acetic Acid) dan BAP (6- Benzil Amino Purine) serta Air Kelapa untuk Menginduksi Organogenesis Tanaman Anthurium (Anthurium andraeanum Linden ex Andre). Bul. Agron. 32(1): 20-25.)

Rahardja, P. C., dan Wahyu, W. 2003. Aneka Cara Memperbanyak Tanaman. Agromedia Pustaka, Jakarta.

Raini, M. dan Isnawati, A. 2011. Kajian: Khasiat dan Keamanan Stevia Sebagai Pemanis Pengganti Gula. Media Litbang Kesehatan Volume 21 Nomor 4 Tahun 2011: 145-156.

Raini, M. dan Isnawati, A. 2011. Kajian: Khasiat dan Keamanan Stevia Sebagai Pemanis Pengganti Gula. Media Litbang Kesehatan Volume 21 Nomor 4 Tahun 2011: 145-156.

Salisbury, F.B., dan Ross, C.W. 1995. Fisiologi Tumbuhan Jilid 2. ITB Press, Bandung.

Sari, D.I. 2014. Empat Teknik Perbanyakan Stevia. Surabaya: BBPPTP.

Singh, S. D. and Rao, G. P. 2005. Stevia: The herbal sugar of the 21st century. Sugar Technol. 7:17-24.

Staba, E. 2000. Plant Tissue Culture as a Source of Biochemical. Florida: CRC Press. Sekaran T, Giridhar P, Ravishankar GA (2007). Production of steviosides in ex vitro and in vitro grown Stevia rebaudiana Bertoni. J. Sci. Food Agric. 87:420-424.

Starratt, A. N., C. W. Kirbyb, R. Pocsa and J. E. Brandle. 2002. Rebaudioside F, a diterpene glycoside from Stevia rebaudiana. Phytochemistry 59:367.

Wetherell, D.F. 1982. Pengantar Propagasi Tanaman secara In Vitro. IKIP Semarang Press, Semarang.

Zulkarnain. 2011. Kultur Jaringan Tanaman. Bumi Aksara, Jakarta.




DOI: http://dx.doi.org/10.33512/j.agrtek.v7i2.1080

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Jurnal Agroekoteknologi

 

INDEXED BY:

      

 

   crossref-logo-landscape-200 


View My Stats Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.