Pengaruh Konsentrasi Benzyl Amino Purin dan Indole Acetic Acid dalam Multiplikasi Tunas Pisang Merah (Musa acuminata Red Dacca)  Asal Banten Secara in Vitro

Irfan Anshori; Sulastri Isminingsih; Nurmayulis Nurmayulis; Susiyanti Susiyanti

doi:10.33512/jur.agroekotetek.v14i2.17326

Pengaruh Konsentrasi Benzyl Amino Purin dan Indole Acetic Acid dalam Multiplikasi Tunas Pisang Merah (Musa acuminata Red Dacca) Asal Banten Secara in Vitro

Irfan Anshori, Sulastri Isminingsih, Nurmayulis Nurmayulis, Susiyanti Susiyanti

Abstract

This study aimed to determine the response of red banana shoots (Musa acuminata Red Dacca) origin from Banten in vitro due to administration of benzyl amino purin (BAP) and indole acetic acid (IAA) in various concentrations. This research was conducted at the Laboratory of Biotechnology and Plant Physiology, University of Sultan Ageng Tirtayasa from March 2022 to May 2022. The research design used was a factorial randomized completely block design consisting of two factors. The first factor is the concentration of BAP which consists of theree levels, namely 0 mg kg-1, 2 mg kg and 4 mg kg-1. The second factor is the concentration of IAA which consists of theree levels, namely 0 mg kg-1, 0.25 mg kg-1 and 0.50 mg kg-1. The concentration of 2 mg kg-1 BAP gave the best results on the parameters of the number of shoots aged 2 weeks after planting (WAP) (2.06 shoots), 4 WAP (2.38 shoots) and 6 WAP (2.50 shoots), the number of leaves aged 2 WAP (1.34 strands), 4 WAP (1.91 strands), 6 WAP (2.16 strands). The concentration of IAA 0 mg kg-1 gave the best results on the parameter of the number of leaves at 2 WAP (1.36 strands). Indole acetic acid concentration of 0.25 mg kg-1 gave the best results on the parameters of
the number of roots aged 2 WAP (1.50 roots), 4 WAP (1.65 roots) and 6 WAP (1.78 roots). There was no interaction between the two treatments.

Keywords

BAP, IAA, Red banana, Benzyl Amino Purin, Indole Acetic Acid, In Vitro

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References

Ahmed, S.S.A.K., Singh, V. K., Wali, dan Kumari, P. 2014. In Vitro Multiplication of Banana (Musa sp.) cv. Grand Naine. J. Biotechnology. Vol.13 (27): 2696–2703.

Ade, H.W. 2019. Pertumbuhan Tunas Pisang Barangan (Musa acuminate L.) Terhadap Pemberian IAA dan Kinetin secara In Vitro. Skripsi. Universitas Muhammadiah Sumatera Utara. Medan.

Anggraeni, R.U.A. 2020. Respon Pertumbuhan Eksplan Anakan Pisang Tanduk (Musa paradisiaca L.) Dengan Pemberian BAP dan IAA secara In Vitro. Skripsi. Universitas Islam Negeri Sultan Syarif Kasim Riau. Pekanbaru.

Bhosale U.P., Dubhashi S.V., Mali N.S., dan Rathod H.P. 2011. In Vitro Shoot Multiplication in Different Species of Banana. Asian J Plant Sci Res. Vol. 1 (3): 23-27.

Bella, D.R.S., Suminar, E., Nuraini, A., Ismail, A. 2016. Pengujian Efektivitas Berbagai Jenis dan Konsentrasi Sitokinin Terhadap Multiplikasi Tunas Mikro Pisang (Musa paradisiaca L.) secara In Vitro. Jurnal Kultivasi. Vol. 15 (2): 74-80.

Badan Pusat Statistik. 2020. Produksi Tanaman Buah-Buahan 2020. https://www.bps.go.id/indicator/55/62/1/produksi-tanaman-buah buahan.html [14 September 2021].

Eriansyah, M., Susiyanti dan Putra, Y. 2014. Pengaruh Pemotongan Eksplan dan Pemberian Beberapa Konsentrasi Air Kelapa terhadap Pertumbuhan dan Perkembangan Eksplan Pisang Ketan (Musa paradisiaca) secara In Vitro. Jurnal Agrologia. Vol. 3 (1): 54-61.

Hartati, S., Arniputri, R.B., Soliah, L.A., and Cahyono, O. 2017. Effects of Organic Additives and Naphthalene Acetid Acid (NAA) Application on The In Vitro Growth of Black Orchid Hybrid (Coelogyne pandurata Lindley). Bulgarian J of Agricultural Science. Vol.23 (6): 951–957.

Lathyfah, U., dan Endah R.S.D. 2016. Pengaruh Variasi Konsentrasi Indole Acetid Acid (IAA) Terhadap Pertumbuhan Tunas Pisang Barangan (Musa acuminata L. triploid AAA.) Dalam Kultur In Vitro. Bioma. Vol. 5 (1): 32-42.

Pamungkas, S.S.T. 2015. Pengaruh Konsentrasi NAA Dan BAP terhadap Pertumbuhan Tunas Eksplan Tanaman Pisang Cavendish (Musa paradisiaca L.) Melalui Kultur In Vitro. Gontor AGROTECH Science Journal. Vol. 2 (2): 31-45.

Putri, R. R. D., Suwirnem, dan Nasril, N. 2018. Pengaruh Naphthalene Asam Asetat (NAA) Pada Pertumbuhan Akar Pisang Raja Kinalun secara In Vitro. J. Bio. Univ. Andalas. Vol.6 (1): 1–5.

Ramesh, Y., dan Ramassamy, V. 2014. Effect of Gelling Agents in In Vitro Multiplication of Banana var. Poovan. Int. J. Advanced Bio. Research. 4 (3): 308–311.

Rosalina, Y., Laili, S., dan Devi, S., Rudi, S. 2018. Karakteristik Tepung Pisang dari Bahan Baku Pisang Lokal Bengkulu. Jurnal Teknologi dan Manajemen Agroindustri. Vol. 7 (3): 153-160.

Su, Y., Liu, Y., dan Zhang, X. 2011. Auxin cytoknin interaction regulates meristem development. Molecular Plant. Vol.4 (4): 616-625.

Sihotang, S., Kardhinata, E.H., dan Riyanto. 2016. Stimulasi Tunas Pisang Barangan (Musa acuminata L.) secara In Vitro dengan Berbagai Konsentrasi IBA (Indole-3-butyric Acid) dan BA (Benzyladenin). BioLink. Vol.3 (1): 18-30.

Sadat, M.S., Luthfi, A.M.S., dan Hot, S. 2018. Pengaruh IAA dan BAP terhadap Induksi Tunas Mikro dari Eksplan Bonggol Pisang Kepok (Musa paradisiaca L.). Jurnal Agroekoteknologi FP USU. Vol.6 (15): 107- 112.

Saragih, R.R. 2018. Upaya Penurunan Fenolat pada Multiplikasi Tunas Mikro Pisang Kepok (Musa paradisiaca L.). Skripsi. Universitas Sumatera Utara. Medan.

Satria, E. 2020. Multiplikasi Tunas Pisang Raja (Musa sapientum L.) dalam Media Murashige dan Skoog Mengandung Benzyl Amino Purine dan Indole Acetic Acid. Skripsi. Universitas Muhammadiyah Sumatera Utara. Medan.

Triharyanto, E., Retno, B.A., Endang, S.M., dan Ellyvia, T. 2018. Kajian Konsentrasi IAA Dan BAP pada Multiplikasi Pisang Raja Bulu In Vitro dan Aklimatisasinya. Jurnal Agrotech Res J. Vol. 2 (1): 1-5.

Wardhany, K.H. 2014. Khasiat Ajaib Pisang. Rapha Publishing. Yogyakarta.

Wahidah, B.F., dan Hasrul. 2017. Pengaruh Pemberian Zat Pengatur Tumbuh Indole Acetic Acid (IAA) Terhadap Pertumbuhan Tanaman Pisang Sayang (Musa paradisiaca L. Var. Sayang) secara In Vitro. Jurnal Teknosains. Vol.11 (1): 27 – 41.

Yatim, H. 2016. Multiplikasi Pisang Raja Bulu (Musa paradisiaca L. AAB GROUP) pada Beberapa Konsentrasi Benzyl Amino Purine (BAP) secara In Vitro. Fakultas Pertanian, Universitas Tompotika, Luwuk. Jurnal Agroekoteknologi. Vol. 4 (3): 1989-1995.

Zarmiyeni., Mahdiannoor., dan Lisa. 2014. Pertumbuhan Tanaman Pisang Kepok (Musa paradisiaca L.) pada Berbagai Konsentrasi BAP secara In Vitro. Jurnal Sains STIPER Amuntai. Vol.4 (1): 22-26.

DOI: http://dx.doi.org/10.33512/jur.agroekotetek.v14i2.17326