PENGARUH LAMA PENYIMPANAN JAMUR Metarhizium anisopliae TERHADAP MORTALITAS ULAT GRAYAK (Spodoptera litura F.) DI LABORATORIUM

Dewi Hastuti; Tubagus Bahtiar Rusbana; Djamaludin Noor Hidayatullah

doi:10.33512/j.agrtek.v9i1.5041

PENGARUH LAMA PENYIMPANAN JAMUR Metarhizium anisopliae TERHADAP MORTALITAS ULAT GRAYAK (Spodoptera litura F.) DI LABORATORIUM

Dewi Hastuti, Tubagus Bahtiar Rusbana, Djamaludin Noor Hidayatullah

Abstract

This research was aimed to know the effectiveness level of different storage time of the fungus Metarhizium anisopliae to armyworm at Laboratory. The research was conducted at BPTPH Region 1 Banten as a repository of biological agents Metarhizium anisopliae and Soil & Agroklimat Laboratory of Agriculture Faculty, Untirta. This research was carried out from October 2015 until February 2016. Environmental design used Randomized Block Design non factorial, with the treatment was storage time of biological agents Metharizium anispoliae that have three levels, i.e. the storage time for 1, 2 and 3 months. Four testing trials include mortality, LT50 test, conidia test, test the intensity of the attacked and describe the morphology of the fungus-infected larvae Metharizium anisopliae. These results showed that the storage time was highly significant in the mortality of Spodoptera litura Fabricius larvae. Provision storage 1 month-long treatment was the best treatment to kill the larvae of Spodoptera litura Fabricius were infected Metharizium anisopliae fungus than the treatment for 2 and 3 months of storage. In the mortality test, LT50 test and intensity test of attacked larvae of Spodoptera litura Fabricius treatment 1 month storage time showed the best results compared to other treatments. The test of conidia density, treatment of storage time 1 month gave showed the highest.

Keywords: Spodoptera litura F., Metharizium anisopliae fungus, Larvae mortality

Keywords

hama

Full Text:

PDF

References

Abbott, W.S. 1925. A Method of Computing the Effectiveness

of Insecticide. Journal of Economic Entomology 18,

-267.

Baehaki, S.E., dan Noviyanti. 1993. Pengaruh Umur Biakan Metarhizium anisopliae Strain Lokal Sukamandi terhadap Perkembangan Wereng Coklat. hlm.113-124. dalam E. Martono, E., Mahrub, N.S., Putra, dan Y. Trisetyawati (Ed.). Simposium Patologi Serangga I. Universitas Gadjah Mada, Yogyakarta, 12-13 Oktober 1993.

Boucias, D.G., and J.C. Pendland. 1998. Principles of Insect

Pathology. Kluwer Academic Publisher. London.

Brousseau, C., G. Charpentier, and S. Belloncik. 1996. Susseptibility of Spruce Budworm, Choristoneura fumiferana

Clemens, to Destruxins, Cyclodepsipeptidic Mycotoxin

of Metarhizium anisopliae. Journal of

Invertebrata Pathology 68 : 180-182.

Ferron, P. 1985. Fungal Control. Comprehensive Insect Phisiology,

Biochem. Pharmacol. 12 : 313-346.

Gabriel, B., Riyatnoo, P. 1989. Metharizium anisopliae (Meetsch) Sor. Taksonomi, Patologi, Produksi dan Aplikasinya. Proyek Pengembangan Perlindungan Tanaman Perkebunan, Departemen Pertanian, Jakarta.

Haryanta, D., A. Susilo, dan H. Prasetyono. 1993. Pengaruh Dosis Dan Waktu Aplikasi Cendawan Beauveria bassiana terhadap Efektivitas Pengendalian Bubuk Buah Kopi (Hypothenemus hampei). hlm. 249-254. dalam E. Martono, E. Mahrub, N.S. Putra, dan Y. Trisetyawati (Ed.). Simposium Patologi Serangga I. Universitas Gadjah Mada, Yogyakarta, 12-13 Oktober 1993.

Husni, Hasanah, Fardhisa, Ade. 2012. Pengaruh Ekstrak Rimpang Jeringau (Acorus calamus L.) Terhadap Mortalitas Ulat Grayak (Spodoptera litura F.). Jurnal. Universitas Syiah Kuala Darussalam Banda Aceh. Kalshoven, L.G.E. 1981. The Pests of Crops in Indonesia. (Revised by P.A. Van der Laan). Ichtiar Baru, Jakarta.

Norman, D.J., R.J. Henny and J.M.F. Yuen. 1997. Disease Resistance In Twenty Dieffenbachia Cultivars. Hort Science. 32 (4) : 709-710.

Pabbage, M.S., A.M. Adnan & N. Nonci. 2007. Pengelolaan Hama Prapanen Jagung. Hal 274-304 dalam Jagung: Teknik Produksi dan Pengembangan. Pusat Penelitian dan Pengembangan Tanaman Pangan Departemen Pertanian, Bogor.

Pendland, J.C. and D.G. Boucias. 1998. Phagocytosis of Lectin Opsonized Fungal Cells and Endocytosis of the Ligand by Insect Spodoptera Exigua Granular Hemocytes: an Ultrastructural and Immunocytochemical Study. CAB (Abstract) (6)7: 1 p. Prayogo, Y., dan W. Tengkano. 2002a. Pengaruh Media Tumbuh Terhadap Daya Kecambah, Sporulasi dan Virulensi Metarhizium anisopliae (Metchnikoff) Sorokin Isolate Kendalpayak pada Larva Spodoptera litura. SAINTEKS. Jurnal Ilmiah Ilmu-ilmu Pertanian. (9) 4: 233-242.

Prayogo, Y., dan W. Tengkano. 2002b. Pengaruh Umur Larva Spodoptera litura terhadap efektivitas Metarhizium anisopliae isolate Kendalpayak dalam N.R. Nganro, C. Sugandawati, M.

Zairin Jr, A. Basukriadi, A. Tahir, P. Sukardi, I. Sulistyo, B. Subardjo, T. Hardiyati, E. Yuwono, Y.Sistina (Ed.). Majalah Ilmiah

Biologi Biosfera (19)3: 70-

Prayogo, Y., dan W. Tengkano. 2002c. Pengaruh Tempat dan Lama Penyimpanan Suspense Spora Metarhizium anisopliae Terhadap Tingkat Kematian Larva Spodoptera litura. hlm. 259-

dalam K. Mulya, S. Rusli, Supriyadi, E.A. Wikardi, M. Djazuli, E.

Karmawati, D. Manohara, O. Rostiana (Ed.). Prosiding Seminar Nasional dan Pameran Pertanian Organik, Jakarta, 2-3 Juli 2002.

Prayogo, Y., dan W. Tengkano. 2004. Pengaruh Konsentrasi

dan Frekuensi Aplikasi Metarhizium anisopliae isolat Kendalpayak Terhadap Tingkat Kematian Spodoptera litura dalam Sudjatinah, Umiyati, P. Bintoro, P. Widiyaningrum, I.O. Utami (Ed.).

SAINTEKS. Jurnal Ilmiah Ilmu-ilmu Pertanian (10)3:

-216.

Prayogo, Yusmani, Tengkano, Wedanimbi, Marwoto. 2005. Prospek Cendawan Entomopatogen Metarhizium anisopliae Untuk Mengendalikan Ulat Grayak Spodoptera litura pada

Kedelai. Jurnal. Balai Penelitian Tanaman Kacangkacangan

dan Umbi-umbian.

Sumartini, Y. Prayogo, S.W. Indiati, dan S. Hardaningsih. 2001. Pemanfaatan Jamur Metarhizium anisopliae untuk Pengendalian Pengisap Polong (Riptortus linearis) pada Kedelai. hlm. 154-157

dalam S.E. Baehaki, E. Santosa, Hendarsih, S.T. Suryana, N. Widarta, dan Sukrino (Ed.). Simposium Pengendalian Hayati Serangga,

Balai Penelitian Tanaman Padi Sukamandi, 14-15 Maret 2001.

Tanada, Y., and H.K. Kaya. 1993. Insect Pathology. Academic

Press, Inc., California. 666 pp.

Widayat, W., dan D.J. Rayati. 1993a. Hasil Penelitian Jamur Entomopatogenik Lokal Dan Prospek Penggunaannya Sebagai Insektisida Hayati. hlm. 61-74 dalam E. Martono, E. Mahrub, N.S. Putra, dan Y. Trisetyawati (Ed.). Simposium Patologi Serangga I. Universitas Gadjah Mada, Yogyakarta, 12-13 Oktober 1993.

Widayat, W., dan D.J. Rayati. 1993b. Pengaruh FrekuensiPenyemprotan Jamur Entomopatogenik Terhadap Ulat Jengkal (Ectropis Bhurmitra) di Perkebunan Teh. hlm. 91-98 dalam E. Martono, E. Mahrub, N.S. Putra, dan Y. Trisetyawati (Ed.).Simposium Patologi Serangga I. Universitas Gadjah Mada,

Yogyakarta, 12-13 Oktober

DOI: http://dx.doi.org/10.33512/j.agrtek.v9i1.5041