Potensi Antibakteri Ekstrak Spons Laut Koleksi Perairan Grand Watu Dodol Banyuwangi

Dian Sari Maisaroh, Nor Sa'adah, Yahya Abdillah Al Hanif

Abstract


This study aims to the antibacterial activity of sea sponge extract from the waters of Grand Watu Dodol Banyuwangi against the pathogenic bacteria Staphylococcus aureus, Vibrio parahaemolyticus, Escherichia coli and Escherichia coli MDR (Multi Drug Resistant). This study uses an experimental laboratory method. The samples found were 3 sponges and macerated using methanol solvent with 3 repetitions. The results of the sponge extract paste were tested by disc diffusion method against pathogenic bacteria S. aureus, V. parahaemolyticus, E. coli and E. coli MDR. Antibacterial test using a concentration of 10 mg/ml with 2 repetitions. The extraction results obtained from the four samples only 3 that can produce bioactive compound extracts. Antibacterial activity against pathogenic bacteria S. aureus produced the largest inhibition zone obtained by GWD B with an average of 8.89 mm. Pathogen E.coli, the largest inhibition zone obtained by GWD B with an average of 8.90 mm. Pathogen V. parahaemolyticus the largest inhibition zone obtained by GWD A produced an inhibition zone with an average of 7.30 mm. Pathogen E.coli MDR produced the largest inhibition zone obtained by GWD A with the largest average inhibition zone of 7.26 mm.


Keywords


antibacterial; Banyuwangi; extract; sponges

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References


Aristyawan, A.D., Sugijanto N.E., Suciati S. 2017. Potensi Antibakteri dari Ekstrak Etanol Spons Agelas cavernosa. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia IV(1): 39 - 43. DOI: https://doi.org/10.20473/jfiki.v4i12017.39-43

Dewatisari, W. F., L. Rumiyanti, dan Rakhmawati, I. 2018. Rendemen dan Skrining Fitokimia pada Ekstrak Daun Sanseviera sp. Jurnal Penelitian Pertanian Terapan 17(3), 197. DOI: https://doi.org/10.25181/jppt.v17i3.336

Dhinakaran, D.I. and Lipton, A.P. 2012. Antimicrobial Potential of the Marine Sponge Sigmadocia pumila from the South Eastern Region of India. World J. Fish. Mar. Sci. 4(4):344-348. DOI: https://doi.org/10.5829/idosi.wjfms.2012.04.04.6353

Hasanah, N., Sudaryatma, P. E., Razaq, I., Eriawati, N.N., Nugraha, W.A., Kumalasari, H., Anggraeni, P.A.S. dan Dewi, I.A.M.M. 2022. Early Detection of Contamination Vibrio parahaemolyticus and Escherichia coli in Fisheries Product Using Multiplex Polymerase Chain Reaction. Jurnal Sain Veteriner, 40(2), 171. DOI: https://doi.org/10.22146/jsv.73314

Hudzicki, J. 2012. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol Author Information. American Society For Microbiology, December 2009, 1–13. https://www.asm.org/Protocols/Kirby-Bauer-Disk-Diffusion-Susceptibility-Test-Pro [ diakses 1 Augustus 2023]

Jones, W. P., and Kinghorn, A. D. 2006. Extraction of Plant Secondary Metabolites. In Natural Products Isolation. Humana Press, pp. 323–351. DOI: https://doi.org/10.1385/1-59259-955-9:323

Josua, E., Wewengkang, D. dan Suoth, E. 2021. Uji Aktivitas Antibakteri Ekstrak dan Fraksi Spons Liosina paradox dari perairan Pulau Mantehage. Pharmacon Journal, 10(3): 933-939. DOI: 10.35799/pha.8.2019.29386

Maisaroh, D.S., Hanif, Y.A.A., Munir, M. dan Sa’adah, N. 2023. Uji Ekstrak Spons Laut Jenis Ptilocaulis marquezii dari Perairan Kendit sebagai Potensi Antibakteri Escherichia coli. Journal of Marine Research 12 (1):161-166. DOI: https://doi.org/10.14710/jmr.v12i1.36278

Mohamad, H., Rosmiati, Muhammad, T.S.T., Andrian, Y., Bakar, K., Ismail, N., Saidin, J., Latip, J., Musa, J. dan Perenrengi, A. 2017. Potential Secondary Metabolites from Marine Sponge Aaptos aaptos for Atherosclerosis and Vibriosis Tretments. Natural Product Communication, 12(8): 1227-1230. DOI: 10.1177/1934578X1701200819

Napitulu, H.G., Rumengan, I.F.M., Wullur, S., Ginting, E.L., Rimper, J.R.T.S.L. dan Toloh, B.H. 2019. Bacillus sp. Sebagai Agensia Pengurai Dalam Pemeliharaan Brachionus rotundiformis Yang Menggunakan Ikan Mentah Sebagai Sumber Nutrisi. Jurnal Ilmiah Platax 7(1): 158-169. DOI: https://doi.org/10.35800/jip.7.1.2019.22627

Novitasari, A.R., Satyantini, W.H., Andriyono, S. dan Sa’adah, N. 2023. Isolasi dan Identifikasi Bakteri Pengurai Mikroplastik Polyethylene Terephthalate dari Sedimen Ekosistem Mangrove Pasir Putih. Journal of Marine Research 12 (1): 52-60. DOI: https://doi.org/10.14710/jmr.v12i1.37503

Oedjijono, Kusharyati, D. F., dan Hendrati, P. M. 2017. Aktivitas Penghambatan Bakteriosin Bifidobacterium spp. Terhadap Bakteri Multi Drugs Resistant (MDR) Escherichia coli dan Klabsiella pneumonia. Pengembangan Sumber Daya Perdesaan Dan Kearifan Lokal Berkelanjutan, November, 631–640.

Prayitno, D.I. 2018. Identifikasi Molekuler Berbasis 16s rDNABakteri Biopigmen yang Berasosiasi dengan AnemonSp. Jurnal Laut Khatulistiwa, 1 (1): 7-12. DOI: https://doi.org/10.26418/lkuntan.v1i1.24003

Sa’adah, N. 2020. Bakteri Simbion Akar Mangrove Avicennia sp. Sebagai Pendegradasi Pewarna Tekstil. Barakuda 45 Jurnal Ilmu Perikanan dan Kelautan 2 (2): 50-55. DOI: https://doi.org/10.47685/barakuda45.v2i2.91

Savitri, I., Suhendra, L. dan Wartini, N.M. 2017. Pengaruh Jenis Pelarut pada Metode Maserasi Terhadap Karakteristik Ekstrak Sargassum polycystum. Jurnal Rekayasa Dan Manajemen Agroindustri, 5(3): 93–101.

Sukmaningrum, K., Yudistira, A., dan Antasionasti, I. (2021). Uji Aktivitas Antioksidan Ekstrak Etanol Spons (Stylissa sp.) yang Dikoleksi Dari Teluk Manado. Pharmacon, 10(1): 756-761. DOI: https://doi.org/10.35799/pha.10.2021.32773

Trianto, A., Hermawan, I., Suzuka, T. and Tanaka, J. 2011. Two New Cytotoxic Candidaspongiolides from an Indonesian Sponge. ISRN Pharmaceutics, 6p. DOI: 10.5402/2011/852619

Wahyuni, D. T. and Widjanarko, S. B. 2014. Pengaruh Jenis Pelarut dan Lama Ekstraksi terhadap Ekstrak Karotenoid Labu Kuning dengan Metode Gelombang Ultrasonik. Jurnal Pangan dan Agroindustri, 3(2):390-401.

Widyowati, W., Munir, M. dan Maisaroh, D.S. 2023. Uji Potensi Antibakteri Ekstrak Metanol Jeroan dan Daging Teripang Bola (Phyllophorus sp.) terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Journal of Natural Sciences 4(1):39-51. DOI: https://doi.org/10.34007/jonas.v4i1.353

Wijayanti, N.P.A.D., Dewi, L.P.M.K., Astuti, K.W. dan Fitri, N.P.E. 2016. Optimasi Waktu Maserasi untuk Manggis (Garcinia mangostana L.) Rind Menggunakan Pelarut Etil Asetat. Jurnal Farmasi dan Ilmu Kefarmasian Indonesia 3(1), 12-17. DOI: https://doi.org/10.20473/jfiki.v3i12016.12-16




DOI: http://dx.doi.org/10.33512/jpk.v13i2.22352

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