Characterization and Anticancer Activity from Gaharu (Aquilaria malaccensis) Stem Bark Extract

Tarso Rudiana, Elda Suci Yala Merru, Hendrawati Hendrawati, Dede Sukandar

Abstract


Aquilaria malaccensis is a plant species from the Thymeleaceae family. A. malaccensis contains secondary metabolites of phenolic groups, namely benzophenone, chromone, coumarin, xanthone, and flavonoids with various biological activities such as antioxidants and anticancer. This study aims to determine the potential of A. malaccensis stem bark extract as an anticancer and to determine the characteristics of secondary metabolite compounds contained in A. malaccensis stem bark extract. Extraction of the stem bark of A. malaccensis by maceration method in stages using n-hexane, ethyl acetate and methanol as solvents. The anticancer activity test used the microculture tetrazolium (MTT) assay against Murine Leukemia P-388 cells. Compound separation using thin layer chromatograph (TLC) and gravity column chromatography (CC) methods. Determination of chemical compounds using liquid chromatography mass spectroscopy/mass spectroscopy (LCMS/MS). The extracts of n-hexane, ethyl acetate, and methanol each had anticancer activity which was classified as active with an IC50 value of 20.64, 26.61, and 14.49 µg/mL. Based on the results of separation and identification of compounds in methanol extract with TLC, CC, and LCMS/MS, methanol extract contains acetaminophen, aminosamptothesin, isophorone, diethyl phthalate, 3-hydroxy-6H-benzochromen-6-one, isolongifolenic, 2-(2-phenethyl) chromone, 4′,7-dihydroxy-2′,5-dimethoxyisoflavone, 4-hydroxy-3-(1-phenylpropyl)-2H-chromone-2-one; 6-methylchromone-2-carboxylic acid; 3,4-dimethyl-2- phenylmorpholine; and β-calacoren compounds.


Keywords


extraction; gaharu; cancer; chromatography; methanol

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References


Abdillah, A. (2006). Aktivitas antiproliferasi ekstrak air daun sisik naga (Pyrrosia nummularifolia (Sw.) Ching) terhadap sel lestari tumor hela secara in vitro. [Skripsi]. IPB, Bogor.

Afiffudden, S. K., Alwi, H., & Hamid, K. H. (2015). Dtermination of 4’-hydroxyacetanilide in leaves extract of Aquilaria malaccensis by high pressure liquid chromatography. Malaysia: Universitas Teknologi Mara.

Alison, M. (2001). Encyclopedia of Life Sciences. In Cancer Journal Nature Publishing Group (4th ed.).

Alley, M. C., Scudiero, D. A., Monks, A., & Hursey, M. (1988).

Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay. Cancer Research Journal, 48(5), 589–601.

Amalina, Y. (2015). Penetapan kadar flavonoid total dan uji aktivitas antioksidan secara in vitro ekstrak etanol daun gaharu (Aquilaria microcarpa Baill). [Skripsi]. Universitas Lambung Mangkurat, Banjarbaru.

Fathoni, A. (2021). Characterization of Phytochemicals and Chemical Compounds of Patat Leaves (Phrynium capitatum) As Wrapping Materials for Pesor Doclang. EduChemia (Jurnal Kimia Dan Pendidikan), 6(1), 13–25.

Gunasekara, S., Kinghorn, A., Cordell, G., & Farnsworth, S. (1981). Plan anti cancer agent xix. Constituent of Aquilaria malaccensis, Journal Nat. Prod, 44(5), 569–572.

Harfinda, E. M., Normagiat, S., & Hardiansyah, G. (2017). Potensi senyawa metabolit sekunder daun Aquilaria Sp asal kalimantan barat untuk pembuatan teh herbal. Ilmu Pengetahuan dan Teknologi.

Huo, H., Zhu, Z., Pang, D., Li, Y., & Huang, Z. (2015). Seskuiterpen anti neuroinflamasi dari kayu gaharu cina. Fitoterapia, 10(6), 115–121.

Ibrahim, A. H., Al-Rawi, S., Majid, A. M. S., Rahman, N. N. A., Salah, K. M. A., & Kadir, M. O. A. (2011). Separation and fractionation of Aquilaria malaccensis oil using supercritical fluid extraction and tthe cytotoxic properties of the extracted oil. Procedia Food Science, 1(11), 1953–1959.

Ismail, N., Ali, N. A. Z., Jamil, M., Rahiman, M. H. F., Tajuddin, S., & Taib, M. (2013). Differentiating agarwood aol quality using artificial neural network. Malaysia: Universitas Teknologi Mara.

Ito, C., Itoigawa, M., Takakura, T., Ruangrungsi, N., Enjo, F., Tokuda, H., Nishino, H., & Furukawa, H. (2003). Chemical Constituens of Garcinia fusca: Structure Elucidation of Eight New Xanthones and Their Cancer Chemopreventive Activity. The Journal of Natural Products, 66, 200–205.

Jiang, Z., Reddy, D., Ackerman, S. K., Salvesen, J., Li, C., Li, Y., & Sun, X. (2004). Novel Lapachone Compounds and Methods of Use Thereof (Patent No. WO 2004/045557 A2). In United States Patent Aplication (WO 2004/045557 A2).

Kementrian Kesehatan RI. (2013). Riset Kesehatan Dasar (RISKESDAS): Perkembangan Kanker Indonesia. Jakarta: Badan Litbang Kemenkes RI.

Kementrian Kesehatan RI. (2018). Riset Kesehatan Dasar (RISKEDAS): Kanker Leukimia. Jakarta: Badan Litbang Kemenkes RI.

Radzi, N. C., & Kasim, F. A. (2020). Effect of microwave pretreatment

oh gaharu essential oil using hydrodistillation method. Indones. J. Chem, 20(4), 960–966.

Rudiana, T., Fitriyanti, F., & Adawiah, A. (2018). Aktivitas Antioksidan dari Batang Gandaria (Bouea macrophylla Griff). EduChemia (Jurnal Kimia Dan Pendidikan), 3(2), 195.

Rudiana, T., Suryani, N., Indriatmoko, D. D., Yusransyah, Amelia, A., Noviany, & Hadi, S. (2019). Characterization of antioxidative fraction of plant stem Bouea macrophylla Griff. Journal of Physics: Conference Series, 1341.

Wang, S., Yu, Z., Wang, C., Wu, C., Guo, P., & Wei, J. (2018). Chemical Constituents and Pharmacological Activity of Agarwood and Aquilaria Plants. Molecules (Basel, Switzerland), 23(2).

Yang, L., Qiao, L., Xie, D., Yuan, Y., Chen, N., Dai, J., & Guo, S. (2012). 2-(2-Phenylethyl)chromones from Chinese eaglewood. Phytochemistry, 76, 92–97.




DOI: http://dx.doi.org/10.30870/educhemia.v6i2.10983

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