Antibacterial Activity of Brown Macroalgae Lipid (Sargassum duplicatum) to The Methicillin Resistant Staphylococcus aureus (MRSA) And Shigella dysentriae Bacteria

Riong Seulina Panjaitan, Ani Rombe Sarungngu’, Lika Lastri Sitorus

Abstract


Methicillin Resistant Staphylococcus aureus (MRSA) is the main cause of nosocomial infections in burn patientsdue to the occurrence of antibiotic resistance. Dysentery or shigellosis is an infection that causes sores (ulcers) in the large intestine in the middle caused by the bacteria Shigella dysentriae. Some studies show that Sargassum duplicatum has antibacterial abilities. This study aimsto determine the antibacterial activity of Sargassum duplicatum seaweed lipid extract to the bacteria Methicillin Resistant Staphylococcus aureus (MRSA) and Shigella dysentriae bacteria. The method used in this study was the Folch method (using sochletation with a solvent ratio of chloroform:methanol (2:1)) to extract lipids from Sargassum duplicatum. Then using the paper disc diffusion method with positive control of tetracycline. From the extraction results obtained, the lipid levels of Sargassum duplicatum, where the chloroform phase was 0.493% (b / b) and the methanol phase was 2.40% (b / b). The results of the lipid antibacterial activity of Sargassum duplicatum at the pure concentration in the methanol phase with an average concentration of 7.07 mm on the Methicillin Resistant Staphylococcus aureus (MRSA) Bacteria while in the Shigella dysentriae bacteria obtained an average of 7.05 mm and the chloroform phase obtained an average diameter of 9.16 mm on Methicillin Resistant Staphylococcus aureus (MRSA) and in Shigella dysentriae Bacteria an average of 3.99 mm while tetracycline antibiotics amounted to 11.67 mm on Methicillin Resistant Staphylococcus aureus (MRSA) and 18.98 mm in Shigella dysentriae bacteria.

Keywords


Fatty acids; Lipid; MRSA; Sargassum duplicatum; Shigella dysentriae

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References


Alamsyah, H. K., & Widowati, I. (2014). Aktivitas Antibakteri Ekstrak Rumput Laut Sargassum cinereum (J.G. Agardh) Dari Perairan Pulau Panjang Jepara Terhadap Bakteri Escherichia coli Dan Staphylococcus epidermidis. Journal of Marine Research, 3(2), 69–78.

Andrew, p. D., & Valerie, j. S. (2010). Antibacterial free fatty acids : activities , mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology, 85(6), 1629–1642. https://doi.org/10.1007/s00253

Arumugham,Suresh.,Ramasamy,Praveenkumar.,Ramasamy,Thangaraj.,Felix,Lewis,Oscar.,Edachery,Baldev.,Dharumadurai,Dhanasekaran and Nooruddin, T. (2014). Microalgal fatty acid methyl ester a new source of bioactive compounds with antimicrobial activity. Journal of Tropical Disease, 4, S979–S984. https://www.sciencedirect.com/science/article/pii/S2222180814607696

Asfar, N. W. (2015). Uji Toksisitas Akut Alga Coklat ( Sargassum sp) Terhadap Mencit ( Mus muscullus). 11, 14.

Ayuning Putri M, Herawati D, K. N. (2015). Pengembangan metode analisis antibiotik tetrasiklin dalam hati ayam menggunakan kromatografi cair kinerja tinggi (KCKT). Prosiding Penelitian Sivitas Akademika Unisba (Kesehatan Dan Farmasi), 372–381.

Bangkele, E. Y., & Greis, S. (2015). Efek Anti Bakteri Dari Ekstrak Lengkuas Putih ( Alpinia galangal [ L ] Swartz ) Terhadap Shigella dysenteriae Healthy Tadulako Journal ( Elli Yane B , Nursyamsi , Silvia Greis : 52-60 ) Disentri basiler atau shigellosis merupakan suatu penyakit infeksi ya. Jurnal Kesehatan Tadulako, 1(2), 52–60. http://jurnal.untad.ac.id/jurnal/index.php/HealthyTadulako/article/viewFile/5737/4503

Davis, W. W. dan T. R. S. (1971). Disc plate methods of microbiological antibiotic assay. Microbiology, 22, 659–665.

Dewi, A. K. (2013). Isolasi, Identifikasi dan Uji Sensitivitas Staphylococcus aureus terhadap Amoxicillin dari Sampel Susu Kambing Peranakan Ettawa (PE) Penderita Mastitis Di Wilayah Girimulyo, Kulonprogo, Yogyakarta. American Journal of Public Health, 31(2), 0126–0421. https://doi.org/10.2105/ajph.45.9.1138

Dharmananda, S. (2002). The nutritional and medicinal value of seaweeds used in Chinese medicine.

Kemenkes Ri. (2013). Riset Kesehatan Dasar; RISKESDAS.

Kemer, K., Paransa, D. S. J., Rumengan, A. P., & Mantiri, D. M. H. (2015). Antibakteri Dari Beberapa Ekstrak Pada Alga Coklat. Jurnal LPPM Bidang Sains Dan Teknologi, 2(2000), 73–81.

Renhoran, M. (2012). Aktivitas antioksidan dan antimikroba Sargassum polycystum. 77. http://repository.ipb.ac.id/bitstream/handle/123456789/63631/C12mre.pdf?sequence=6&isAllowed=y

Ryantin Gunawan, E., & Suhendra, D. (2012). Screening Dan Analisis Kadar Omega-3 Dari Rumput Laut Pulau Lombok Ntb. Molekul, 7(2), 95. https://doi.org/10.20884/1.jm.2012.7.2.111

Saputro, I., Zarasade, L., & Prasanti, R. (2016). Hambatan Kolonisasi Methicillin Resistant Staphylococcus Aereus oleh Ekstrak Kulit Delima pada Luka Bakar Derajat-2 pada Tikus (Inhibition Of Methicillin Resistant Staphylococcus Aureus Bacteria Colonization On Second Degree Burn Wound In Wistar Rat By US. Jurnal Veteriner, 17(3), 418–423. https://doi.org/10.19087/jveteriner.2016.17.3.418

Sarabashi, S. (2010). Anatomy of the Skin. Dalam: Sarabashi S(Ed). (1st ed.). Jaypee Brothers Medical Publisher.




DOI: http://dx.doi.org/10.30870/educhemia.v5i1.6432

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