Dyhydrokaempferol-3-O-Ramnoside from Ethyl Acetate Extract Ki Encok (Plumbago zeynalica L.) Leaves

Nani Suryani, Euis Julaeha, Tati Herlina

Abstract


Plumbago zeynalica L. plant belongs to the Plumbaginaceae family, which is spread as a weed in all tropical and sub-tropical countries, and its abundance is very high in Asia and Africa. P. zeynalica L. plants are ethnopharmacologically used as antitumor, antioxidant, antimicrobial, anticancer, wound healed, and anti-inflammatory. This study aims to isolate secondary metabolites from the fraction of P. zeynalica L. leaf extracts with work stages that included isolation and determination of chemical structures by spectroscopy. Based on the results of the separation and purification of the ethyl acetate fraction obtained isolates in the form of a yellow oil suspected as a flavonoid. The chemical structure of the isolates was identified based on UV-Vis spectroscopy data giving maximum absorption at λmax 339.60 nm and 278.80 nm, which are typical for dihydroflavonol. IR spectroscopy analysis of the isolates provided data information on the presence of aliphatic OH, C-H groups, carbonyl groups (C = O), C = C aromatic groups, and ether strain (C-O-C). NMR data showed that the isolate had the molecular formula of C21H22O10, and its molecular weight confirmed by MS spectrometer was obtained m/z 434. Isolates from this ethyl acetate fraction were suspected as dyhydrokaempferol-3-O-ramnoside or engeletin.


Keywords


Plumbago zeynalica; flavonoid; dyhydrokaempferol-3-O-rhamnoside; engeletin

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References


Ali, A. H., Hassan, N. M., Shukor, N. I., Embi, N., Latip, J., & Mohd Sidek, H. M. (2014). Anti-plasmodial activity of engeletin isolated from Artocarpus scortechinii. Malaysian Applied Biology, 43(1).

Beyene, B. B., Alem, F. A., & Ayana, M. T. (2020). Determination of antioxidant and antibacterial activities of leaf extracts of Plumbago zeylanica (Amira) . Cogent Chemistry, 6(1), 1831715. https://doi.org/10.1080/23312009.2020.1831715

Brown, G. D., Bauer, J., Osborn, H. M. I., & Kuemmerle, R. (2018). A Solution NMR Approach to Determine the Chemical Structures of Carbohydrates Using the Hydroxyl Groups as Starting Points. ACS Omega, 3(12), 17957–17975. https://doi.org/10.1021/acsomega.8b02136

Chen, L., Yin, H., Lan, Z., Ma, S., Zhang, C., Yang, Z., Li, P., & Lin, B. (2011). Anti-hyperuricemic and nephroprotective effects of Smilax china L. Journal of Ethnopharmacology, 135(2). https://doi.org/10.1016/j.jep.2011.03.033

Cong, H. J., Zhang, S. W., Shen, Y., Zheng, Y., Huang, Y. J., Wang, W. Q., Leng, Y., & Xuan, L. J. (2013). Guanidine alkaloids from Plumbago zeylanica. Journal of Natural Products, 76(7), 1351–1357. https://doi.org/10.1021/np400235s

Dhalani, J., Dubal, G., Rathod, C., & Nariya, P. (2020). Evaluation of Non-polar Composition in Plumbago Zeylanica Leaves by Gas Chromatography and Mass Spectrometry. Folia Medica, 62(2), 308–313. https://doi.org/10.3897/folmed.62.e47647

Ganesan, K., & Gani, S. (2013). Ethnomedical and Pharmacological Potentials of Plumbago zeylanica LA. American Journal of Phytomedicine and Clinical Therapeutics, 1(3), 313–337. http://www.ajpct.org/PA-400163-[6].pdf

Huang, H., Cheng, Z., Shi, H., Xin, W., Wang, T. T. Y., & Yu, L. (2011). Isolation and characterization of two flavonoids, engeletin and astilbin, from the leaves of Engelhardia roxburghiana and their potential anti-inflammatory properties. Journal of Agricultural and Food Chemistry, 59(9), 4562–4569. https://doi.org/10.1021/jf2002969

Kapare, H. S., Metkar, S. R., & Shirolkar, S. (2020). Anticancer Potential Of Plumbago Zeylanica Linn . And Its Isolated Constituent Plumbagin : A Review ’. International Journal of Pharmaceutical Sciences and Research, 11(October). https://doi.org/10.13040/IJPSR.0975-8232.11(10).4859-65

Kumar, S., & Pandey, A. K. (2013). Chemistry and Biological Activities of Flavonoids: An Overview. TheScientificWorldJournal, 2013, 162750. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3891543&tool=pmcentrez&rendertype=abstract

Parveen, M., & Ghalib, R. M. (2012). Flavonoids and antimicrobial activity of leaves of Xylosma longifolium. Journal of the Chilean Chemical Society, 57(1), 989–991. https://doi.org/10.4067/S0717-97072012000100007

Roy, A., & Bharadvaja, N. (2017). A review on pharmaceutically important medical plant: Plumbago zeylanica. Journal of Ayurvedic and Herbal Medicine, 3(4), 225–228. www.ayurvedjournal.com

Ruangnoo, S., Jaiaree, N., Makchuchit, S., Panthong, S., Thongdeeying, P., & Itharat, A. (2012). An in vitro inhibitory effect on RAW 264.7 cells by antiinflammatory compounds from Smilax corbularia Kunth. Asian Pacific Journal of Allergy and Immunology, 30(4), 268–274.

Syahid S.F & Nova K.N. (2016). Multiplikasi Tunas, Aklimatisasi Dan Analisis Mutu Simplisia Daun Encok (Plumbago zeylanica L.) Asal Kultur In Vitro Periode Panjang. Buletin Penelitian Tanaman Rempah Dan Obat, 19(2), 117–128. https://doi.org/10.21082/bullittro.v19n2.2008.%p




DOI: http://dx.doi.org/10.30870/educhemia.v6i1.9025

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