In Silico Study and Bioactivity of Flavonoid Extract Syzygium polyanthum (Wight) Walp. Leaves Against Salmonella typhi

Agus Rochmat, Anisa Hidayati, Mujijah Mujijah, suhaedah suhaedah

Abstract


Salmonella typhi bacteria cause complications of bleeding in the intestine or intestinal perforation. However, Salmonella typhi bacteria will become resistant to chloramphenicol due to the formation of plasmids that produce the chloramphenicol acetyl transferase (CAT) enzyme which works to activate chloramphenicol. So, the search for active groups of natural compounds which are expected to have the work of deactivating the CAT enzyme. One of them is a flavonoid compound. The flavonoid group is widely used as an antimicrobial. This research was aimed at the extraction of bay leaf flavonoids. The presence of flavonoids was proven by phytochemical tests, namely a yellow color change with a slightly acidic amyl alcohol solvent - Mg metal and strengthened by the scanning results of the maximum wavelength on the band 1 407 nm and band 2 338 nm which is a special feature of flavonoid backbone. The results of GC-MS analysis obtained 4 flavonoid compounds from bay leaf extract which have an abundance above 1% and qualification above 90%: 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, alpha- tocopherol, beta-tocopherol, and pyragallol. The correlation between the results of the inhibitory test against Salmonella typhi at a concentration of 100 ppm with the results of docking tocopherol - 3CLA receptors which have an affinity bond energy / ∆Gbinding = 7.40 kcal / mol, indicates that the bay leaf flavonoid extract can inhibit the formation of chloramphenicol acetyl transferase (CAT).


Full Text:

PDF

References


Paul UK, Bandyopadhyay A. Typhoid fever: a review. Int J Adv Med. 2017;4(2):300.

Ugboko H, De N. Mechanisms of Antibiotic resistance in Salmonella typhi. IntJCurrMicrobiolAppSci [Internet]. 2014;3(12):461–76. Available from: http://www.ijcmas.com

Wain J, Hendriksen RS, Mikoleit ML, Keddy KH, Ochiai RL. Typhoid fever. Lancet [Internet]. 2015;385(9973):1136–45. Available from: http://dx.doi.org/10.1016/S0140-6736(13)62708-7

Cita YP. Bakteri Salmonella typhi dan demam tifoid. J Kesehat Masy Sept - Maret 2011. 2011;6(1):42–6.

Maulana Y. SENSITIVITAS Salmonella Sp. PENYEBAB DEMAM TIFOID TERHADAP BEBERAPA ANTIBIOTIK DI RUMAH SAKIT IMMANUEL BANDUNG. Maj Kedokt Bandung. 2009;41(3).

Kusuma IW, Kuspradini H, Arung ET. Biological Activity and Phytochemical Analysis of Three Indonesian Medicinal Plants , Murraya koenigii , Syzygium polyanthum and Zingiber purpurea. J Acupunct Meridian Stud [Internet]. 2011;4(1):75–9. Available from: http://dx.doi.org/10.1016/S2005-2901(11)60010-1

Trisharyanti I, Febriani R, Farmasi F, Muhammadiyah U. SKRINING AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN TERHADAP Salmonella Typhi. J Pharm Sci Clin Res [Internet]. 2017;02:66–77. Available from: https://jurnal.uns.ac.id/jpscr/issue/current/showToc

Evendi A. UJI FITOKIMIA DAN ANTI BAKTERI EKSTRAK DAUN SALAM ( Syzygium polyanthum ) TERHADAP BAKTERI Salmonella typhi DAN Escherichia coli SECARA IN VITRO Agus Evendi Analis Kesehatan , Poltekkes Kemenkes Kaltim , Jl . Kurnia Makmur No . 64 Abstrak PENDAHULUAN Indo. Mahakam Med Lab Technol J. 2017;II(1):1–9.

Ghasemzadeh A, Ghasemzadeh N. Flavonoids and phenolic acids : Role and biochemical activity in plants and human Figure 1 . Basic structure of flavonoids . 2011;5(31):6697–703.

Rizki AU, AR C, Amalia M. Perbedaan Efektivitas Ekstrak... (Ayu et.al). J Profesi Med J Kedokt dan Kesehat. 2016;10(1):54–69.

Rochmat A. Karakteristik Senyawa Flavonoid Ekstrak Sambiloto (Andrographis paniculata) yang Mempunyai Aktivitas Inhibisi TErhadap Enzim Siklooksigenase-2 Secara In Vitro. J Integr PROSES. 2015;5(2):81–7.

Balamurugan V, Sheerin FMA, Velurajan S. A GUIDE TO PHYTOCHEMICAL ANALYSIS. Int J Adv Res Innov Ideas Educ. 2019;5(January):236–45.

Harborne JB. Phytochemical Methods A Guide To Modern Tecniques Of Plant Analysis, Third Edition. Third. Vol. Third Edit, Chapman & Hall. London: Chapman and Hall; 1998. 1–217 p.

Rochmat A. PENGEMBANGAN SALEP LUKA BAKAR EKSTRAK FLAVONOID DAUN SRIKAYA (Annona squamosa L). Sci J Farm dan Kesehat. 2018;8(1):1.

Somia Gu, Asma Eraj ZA. Glycyrrhiza glabra and Azadirachta indica against Salmonella Typhi: Herbal Treatment as an Alternative Therapy for Typhoid Fever. Arch Med. 2015;7(6):11–5.

Herdiawan B, Rulitasari D, Aprilianty ET, Khalisah H, Choirudiniyah NF, Imtiyaz JD, et al. In Silico Potential of Turmeric (Cucurma longa) as Antibacterial Agent for Salmonella typhi. Bioedukasi. 2018;XVI(2):103.

Samykannu G, Vijayababu P, Natarajan J. Informatics in Medicine Unlocked Substrate specificities in Salmonella typhi outer membrane protease ( PgtE ) from omptin family – An in silico proteomic approach. Informatics Med Unlocked [Internet]. 2019;16(August):100237. Available from: https://doi.org/10.1016/j.imu.2019.100237

Suresh X. In silico studies on structure prediction and inhibitory action of selected natural compounds on cell invasion protein SipB from Salmonella Typhi. Asian J Biomed Pharm Sci. 2014;4(32):20–4.

Chemistry, Biochemistry and Applications.

Kesehatan M. UJI SITOTOKSIK KOMBINASI CISPLATIN DENGAN EKSTRAK ETANOL BENALU ALPUKAT ( Dendrophthoe pentandra ( L ) Miq .) PADA SEL HELA Roihatul Mutiah *  , Arief Suryadinata *, Prasasti Swara Nurani * Abstrak CYTOTOXIC EVALUATION OF CISPLATIN IN COMBINATION WITH ET. 2018;5(September):133–43.

Sandika J, Suwandi JF. Sensitivitas Salmonella thypi Penyebab Demam Tifoid terhadap Beberapa Antibiotik. Majority. 2017;6(1):41–5.

Sidabutar S, Satari HI. Pilihan Terapi Empiris Demam Tifoid pada Anak: Kloramfenikol atau Seftriakson? Sari Pediatr. 2016;11(6):434.

Sugijanto NE, Anggraeny D, Zaini NC. Aktivitas Antimikroba Ekstrak Jamur Endofit Kabatiella caulivora var B yang diisolasi dari Alyxia reinwardtii. J Ilmu Kefarmasian Indones. 2011;9(1):31–4.

Nafsa G, Mutmaina, Sopinah S. UJI ANTIBAKTERI EKSTRAK ETANOL DAUN SALAM ( Syzygium polyanthum ) DATARAN TINGGI DAN RENDAH TERHADAP PERTUMBUHAN Salmonella sp . Pros Semin Nas dan Disem Penelit Kesehatan STIKes Bakti Tunas Husada Tasikmalaya, 21 April 2018. 2018;(April):212–5.

Clinical and Laboratory Standards Institute C. M02-A12: Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Twelfth Edition. Clin Lab Stand Inst. 2015;35(M02-A12):73.

Ali MT, Blicharska N, Shilpi JA, Seidel V, Dash P, Bala Divya M, et al. Discovery of Benzothiazole Scaffold-Based DNA Gyrase B Inhibitors. Sci Rep [Internet]. 2020;9(1):1–10. Available from: http://arxiv.org/abs/2007.00621

Leslie AGW. Refined crystal structure of type III chloramphenicol acetyltransferase at 1·75 Å resolution. J Mol Biol. 1990;213(1):167–86.

Antao T. Bioinformatics with Python Cookbook. Livro. 2015. 306 p.

Granchi C, Capecchi A, Del Frate G, Martinelli A, Macchia M, Minutolo F, et al. Development and validation of a docking-based virtual screening platform for the identification of new lactate dehydrogenase inhibitors. Molecules. 2015;20(5):8772–90.

Deligkaris C, Ascone AT, Sweeney KJ, Greene AJQ. Validation of a computational docking methodology to identify the non-covalent binding site of ligands to DNA. Mol Biosyst. 2014;10(8):2106–25.




DOI: http://dx.doi.org/10.62870/wcej.v4i2.10593

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 World Chemical Engineering Journal

WCEJ (e-ISSN: 2443-2261) is published by Chemical Engineering Department, Universitas Sultan Ageng Tirtayasa (UNTIRTA).

This Journal has been indexed by:

  1. Google Scholar
  2. Garuda
  3. Dimensions
  4. Crossref
  5. Open Academic Journal Index
  6. Journal Impact Factor
  7. Cite Factor 
Archives: Resources | Dimensions CrossrefJIFACTOR, Journal Indexing  

 

 

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.