Kajian Kombinasi Perlakuan Zat Pengatur Tumbuh TDZ dan Benzil Adenin Terhadap Perkembangan Kalus Durian Merah

Khoirul Bariyyah; Putri Istianingrum

doi:10.33512/jur.agroekotetek.v13i1.12161

Kajian Kombinasi Perlakuan Zat Pengatur Tumbuh TDZ dan Benzil Adenin Terhadap Perkembangan Kalus Durian Merah

Khoirul Bariyyah, Putri Istianingrum

Abstract

Red durian plants have the potential to be developed because they have high economic value. Red durian plants are propagated conventionally in two ways, namely through seeds and shoot grafts. Propagation using shoot graft creates a new problem for red durian farmers, namely a decrease in fruit production on the parent plant due to large amounts of scion. One alternative to the propagation of red durian seeds without damaging the parent plant can be done by using tissue culture techniques. The explants used in this study was durian callus. The callus was grown on B5 media treated with a plant growth regulator combination in the form of TDZ consisting of six treatments, namely 0, 0.4, 0.8, 1.2, 1.6 ppm and BA consisting of five treatment, namely 0, 0.3, 0.6, 0.9, 1.2 ppm. This research was conducted to determine the best dose of combination treatment of tidiazuron (TDZ) with BA in the formation of shoots from red durian callus through tissue culture. The results showed that at the age of 6 months after culture, red durian callus had a color change from yellow to green in the TDZ treatment 1.6 ppm + BA 1.2 ppm.

Keywords

culture, callus, red durian

Full Text:

PDF

References

Daud, N.H., S. Jayaraman dan R. Mohamed. 2012. Methods Paper: An improved surface sterilization technique for introducing leaf, nodal and seed explants of Aquilaria malaccensis from field sources into tissue culture. Aspac J Mol Biol Biotechnol, 20(1) : 55 – 58.

Fauziyyah, D., T. Hardiyati, dan Kamsinah. 2012. Upaya Memacu Pembentukan Kalus Eksplan Embrio Kedelai (Glycine Max (L.) Merril) Dengan Pemberian Kombinasi 2.4-D dan Sukrosa Secara Kultur In Vitro. Jurnal Pembangunan Pedesaan 12 (I) : 30 - 37

Flick, C.E., D.A. Evans, and W.R. Sharp. 1993. Organogenesis. In D.A. Evans, W.R.Sharp, P.V. Amirato, and T. Yamada (eds.) Handbook of Plant Cell Culture Collier Macmillan. Publisher London. p. 13-81.

Gaba, V.P. 2005. Plant Growth Regulator. In R.N. Trigiano and D.J. Gray (eds.) Plant Tissue Culture and Development. CRC Press. London. p. 87-100.

Hartati, H. Agani, N. S. Hartati, E. Sudarmonowati. 2018. Kecepatan Regenerasi Kalus Somatik Embriogenik TerungPada Beberapa Media. Jurnal Ilmu Dasar 9 (2) :125-134.

Husni A. 2005. Regenerasi Protoplas Tanaman Terung dan Ketahanan Regeneran Terhadap Penyakit Bakteri Layu. Berita Biologi. 7: 285-292.

Lestari, E.G. 2011. Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal AgroBiogen 7(1):63-68

Mng’omba, S.A., G. Sileshi dan E.S. du Toit. 2012. Efficacy and Utilization of Fungicides and Other Antibiotics for Aseptic Plant Cultures. In D. Dhanasekaran (Ed.), Fungicides for Pland and Animal Diseases (pp. 245–254). InTech Europe. https://doi.org/10.5772/1130.

Oratmangun, K.M., D. Pandiangana dan F.E. Kandou. 2017. Deskripsi Jenis-jenis Kontaminan dari Kultur Kalus Catharanthus roseus (L.) G. Don. Jurnal MIPA UNSRAT Online, 6(1) : 47 – 52.

Oyebanji, O.B, O. Nweke, O. Odebunmi, N.B. Galadima, M.S. Idris, U.N. Nnodi, A.S. Afolabi dan G.H. Ogbadu. 2009. Simple, effective and economical explantsurface sterilization protocol for cowpea, rice and sorghum seeds. Afr J. Biotechnol 8(20) : 5395-5399.

Rosyidah, M., Evie, R., dan Yuni, S.R. 2014. Induksi Kalus Daun Melati (Jasminum sambac) dengan Penambahan Berbagai Konsentrasi Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylamino Purin (BAP) pada Media MS secara In Vitro. Jurnal Biologi. Vol 3(3). Hal : 147-153.

Rusmiati, E. Mulyono, S. Ashari, M.A. Widodo, dan L. Basir. 2013. Eksplorasi, Inventarisasi dan Karakterisasi Durian Merah Banyuwangi.online. https://jurnal.fmipa.unila.ac.id/semirata/article/view/622/442. Diakses pada 27 Oktober 2020.

Sugito, H., Y. Santosa, E. Sandra. 2006. Penggunaan Thidiazuron, 2, 4 – D Dan Giberellin Dalam Pembentukan Embrio Somatik Pule Pandak (Rauvolfia Serpentina (L.) Benth. Ex Kurz) Melalui Kultur In Vitro. Media Konservasi Vol. Xi, No. 2 : 66-71.

Tajuddin, T., Karyanti, T. Sukarnih, N. Haska. 2014. A revised method for sucker sterilization to support the in vitro propagation of sago palm (Metroxylon sagu Rottb.). J. Bioteknol Biosains Indonesia, 1(1) : 21 – 26. doi: 10.29122/jbbi.v1i1.548.

Widyawati, G. 2010. Pengaruh Variasi Konsentrasi NAA dan BAP tehadap Induksi Kalus Jarak Pagar (Jatropha curcas L. Tesis. Biosains. Universitas Sebelas Maret. Surakarta.

Zaer and Mapes. 1982. Action of growth regeneration. In Bonga and Durzan (eds.) Tissue Culture in Forestry. Martinus Nijhoff London. p. 231-235.

DOI: http://dx.doi.org/10.33512/jur.agroekotetek.v13i1.12161