Pengujian Biologis Formulasi Bioenkapsulasi Bacillus sp. untuk Menghambat Penyakit Layu Bakteri pada Tanaman Cabai

Mochammad Mirza Saputra; Yenny Wuryandari; Noni Rahmadhini

doi:10.33512/jur.agroekotetek.v16i1.23213

Pengujian Biologis Formulasi Bioenkapsulasi Bacillus sp. untuk Menghambat Penyakit Layu Bakteri pada Tanaman Cabai

Mochammad Mirza Saputra, Yenny Wuryandari, Noni Rahmadhini

Abstract

Bacterial wilt disease is a serious threat to cayenne pepper plants because it can cause a significant reduction in production. Although Bacillus sp. has been applied using liquid formulations, the results have not been optimal. Therefore, bioencapsulated formulations in the form of Beads have been tested as an alternative to increase their effectiveness. These Beads were made using sodium alginate cross-linked with CaCl2 through an extrusion technique. The application of Beads with the K2W1 treatment combination showed the lowest disease intensity level, which was 55.5%, and the effectiveness of inhibition reached 44.5%. From the results of this study, it can be concluded that the beads formulation is significantly potentian in inhibiting bacterial wilt disease on chili plant

Keywords

Bacillus sp.; Bacterial Wilt; Bioencapsulation; Beads;

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References

Abidin, Z., LQ, A., & AL, A. (2015). Pengaruh bakteri Bacillus spp. dan Pseudomonas sp. Terhadap pertumbuhan cendawan patogen S. rolfsii Sacc.penyebab penyakit rebah semai pada tanaman kedelai. Jurnal HPT, 3(1), 1–10.

Anjarsari, D. T., Prasetyawati, E. T., & Wuryandari, Y. (2022). Inhibitory Test of Bacillus sp. Against Phytophthora palmivora Causes Cocoa Fruit Rot Disease. Nusantara Science and Technology Proceedings, 14–21. https://doi.org/10.11594/nstp.2022.2003

Aoki, K., Wang, B., Chen, J., & Nishiumi, T. (2012). Diffusion coefficients in viscous sodium alginate solutions. Electrochimica Acta, 83, 348–353.

Arwiyanto, T., & Hartana, I. (1999). Pengendalian hayati penyakit layu bakteri tembakau, percobaan rumah kaca. Jurnal Perlindungan Tanaman Indonesia, 5, 50–59.

Asril, M., Lisafitri, Y., & Siregar, B. A. (2020). A Possibility of Proteolytic Bacteria Utilization to Control Ralstonia solanacearum 59 In Vitro. IOP Conference Series: Earth and Environmental Science, 537(1), 012040. https://doi.org/10.1088/1755-1315/537/1/012040

Bevilacqua, A., Campaniello, D., Speranza, B., Racioppo, A., Altieri, C., Sinigaglia, M., & Corbo, M. R. (2020). Microencapsulation of Saccharomyces cerevisiae into Alginate Beads: A Focus on Functional Properties of Released Cells. Foods, 9(8), 1051. https://doi.org/10.3390/foods9081051

Chi, Y., Wang, D., Jiang, M., Chu, S., Wang, B., Zhi, Y., Zhou, P., & Zhang, D. (2020). Microencapsulation of Bacillus megaterium NCT-2 and its effect on remediation of secondary salinization soil. Journal of Microencapsulation, 37(2), 134–143. https://doi.org/10.1080/02652048.2019.1705409

Choliq, F. A., Martosudiro, M., Istiqomah, I., & Fanhash Nijami, M. (2020). Isolasi Dan Uji Kemampuan Bakteriofag Sebagai Agens Pengendali Penyakit Layu Bakteri (Ralstonia solanacearum) Pada Tanaman Tomat. VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian, 14(1), 8–20. https://doi.org/10.35457/viabel.v14i1.996

Essifi, K., Brahmi, M., Berraaouan, D., Ed-Daoui, A., El Bachiri, A., Fauconnier, M.-L., & Tahani, A. (2021). Influence of Sodium Alginate Concentration on Microcapsules Properties Foreseeing the Protection and Controlled Release of Bioactive Substances. Journal of Chemistry, 2021, e5531479. https://doi.org/10.1155/2021/5531479

Fakhruddin, D. K. (2020). Viabilitas Bacillus sp. Sebagai Agen Antagonis Patogen TanamanDalam Formulasi Berbahan Dasar Tepung. Skripsi. Universitas Jember.

Irjayanti, A. D., Wibowo, A. S., Sumartini, N. P., Nurfalah, Z., Adani, A. D., Sijabat, M. S., Situmorang, N., Iman, Q., Damayanti, S. R., & Putra, Y. R. (2021). Statistik Hortikultura 2021. Badan Pusat Statistik.

Istiqomah, I., & Kusumawati, D. E. (2018). Pemanfaatan Bacillus subtilis dan Pseudomonas fluorescens dalam pengendalian hayati Ralstonia solanacearum penyebab penyakit layu bakteri pada tomat. Jurnal AGRO, 5(1), Article 1. https://doi.org/10.15575/2305

Kim, I.-Y., Pusey, P. L., Zhao, Y., Korban, S. S., Choi, H., & Kim, K. K. (2012). Controlled release of Pantoea agglomerans E325 for biocontrol of fire blight disease of apple. Journal Control Release, 161(1), 109–115.

Li, L., Feng, X., Tang, M., Hao, W., Han, Y., Zhang, G., & Wan, S. (2014). Antibacterial activity of Lansiumamide B to tobacco bacterial wilt (Ralstonia solanacearum). Microbiological Research, 169(7), 522–526. https://doi.org/10.1016/j.micres.2013.12.003

Li, Z. Q., Hou, L. D., Li, Z., Zheng, W., & Li, L. (2013). Study on Shape Optimization of Calcium–Alginate Beads. Advanced Materials Research, 648, 125–130. https://doi.org/10.4028/www.scientific.net/AMR.648.125

Martínez-Cano, B., Mendoza-Meneses, C. J., García-Trejo, J. F., Macías-Bobadilla, G., Aguirre-Becerra, H., Soto-Zarazúa, G. M., & Feregrino-Pérez, A. A. (2022). Review and Perspectives of the Use of Alginate as a Polymer Matrix for Microorganisms Applied in Agro-Industry. Molecules, 27(13), 4248. https://doi.org/10.3390/molecules27134248

Musafa, M. K., Aini, L. Q., & Prasetya, B. (2015). Peran Mikoriza Arbuskula Dan Bakteri Pseudomonas fluorescens Dalam Meningkatkan Serapan P Dan Pertumbuhan Tanaman Jagung Pada Andisol. Jurnal Tanah Dan Sumberdaya Lahan, 2(2), 191–197.

Parke, J. L. (1991). Root colonization by indigenous and introduced microorganisms. In The Rhizosphere and Plant Growth (pp. 33–42). Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3336-4_4

Pitchaon, M., Tanawan, W., & Thepkunya, H. (2013). Tamarind kernel powder, gum arabic and maltodextrin as a novel combination for encapsulating agents of phenolic antioxidants. International Food Research Journal, 20(2).

Rojas-Padilla, J., de-Bashan, L. E., Parra-Cota, F. I., Rocha-Estrada, J., & de los Santos-Villalobos, S. (2022). Microencapsulation of Bacillus Strains for Improving Wheat (Triticum turgidum Subsp. Durum) Growth and Development. Plants, 11(21), 2920. https://doi.org/10.3390/plants11212920

Sachdev, S., & Singh, R. P. (2018). Root colonization: Imperative mechanism for efficient plant protection and growth. MOJ Ecol Environ Sci, 3(4), 240–242.

Sholeh, A., Yulianah, I., & Purnamaningsih, S. L. (2017). Penampilan Sifat Ketahanan Penyakit Layu Bakteri (Ralstonia solanacearum) Dan Produktivitas Tinggi Tanaman Cabai Merah (Capsicum annuum L.) Pada 24 Famili F5. Jurnal Produksi Tanaman, 5(6), 957–964.

Sianipar, H. F., Sijabat, A., & Pane, E. P. (2019). Pengaruh Pemberian Berbagai Tingkat Mikoriza Arbuskula Pada Tanah terakumulasi Logam Pb terhadap Pertumbuhan tanaman Belimbing Wuluh (Averrhoa bilimbi). JBIO: Jurnal Biosains, 5(2), 53–58.

Sutarti, G. A. K., & Wahab, A. (2010). Isolasi dan Uji Kemampuan Rizobakteri Indigenous sebagai Agensia Pengendali Hayati Penyakit pada Tanaman Cabai. Jurnal Hortikultura, 20(1), 86–95.

Tu, L., He, Y., Yang, H., Wu, Z., & Yi, L. (2015). Preparation and characterization of alginate–gelatin microencapsulated Bacillus subtilis SL-13 by emulsification/internal gelation. Journal of Biomaterials Science, Polymer Edition, 26(12), 735–749.

van Elsas, J. D., Trevors, J. T., Jain, D., Wolters, A. C., Heijnen, C. E., & van Overbeek, L. S. (1992). Survival of, and root colonization by, alginate-encapsulated Pseudomonas fluorescens cells following introduction into soil. Biology and Fertility of Soils, 14(1), 14–22. https://doi.org/10.1007/BF00336297

Zinidin, M. (2022). Eksplorasi Bacillus spp. Pada Rhizosfer Cabai Merah (Capsicum annuum L.) Dataran Tinggi dan Potensinya Sebagai Agensia Pengendali Hayati Patogen Ralstonia Solanacearum Secara In Vitro. Skripsi. Universitas Pembangunan Nasional ‘Veteran’ Jawa Timur.

DOI: http://dx.doi.org/10.33512/jur.agroekotetek.v16i1.23213