Characterization of Phytochemicals and Chemical Compounds of Patat Leaves (Phrynium capitatum) as Wrapping Materials for Pesor Doclang

Ahmad Fathoni

Abstract


Doclang is a typical food of Bogor. One of the compositions is “pesor” wrapped in patat leaves. Apart from being a food wrapper, patat leaves are used as traditional medicine. Research on the chemical content of patat leaves is still limited and needs to be further developed. In this study, phytochemical testing and chemical content of the extracts of n-hexane, ethyl acetate, and methanol from patat leaves were carried out. Phytochemical tests include alkaloid tests; steroid and terpenoid testing; flavonoid test; saponin test; phenolic hydroquinone; tannin and polyphenol test. Based on phytochemical test results, the extract of patat leaves was known to contain alkaloids, steroids, triterpenoids, flavonoids, saponins, phenolic hydroquinones, and tannins with different distributions at each level of solvent polarity. The instruments used to identify the chemical content of patat leaf extract were GCMS and LCMS QTOF. Through GCMS analysis on n-hexane extract, 114 compounds were identified with a similarity level of ≥ 80% to the library. Among these compounds, confirmed to be terpenoid and steroid compounds. In the LCMS analysis of ethyl acetate extract obtained 25 compounds and 31 compounds for methanol extract. It is suspected that these compounds come from the alkaloid, steroid, flavonoid, saponin, phenolic hydroquinone, or tannin class.

Keywords


packaging; phytochemicals; GCMS; LCMS QTOF

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References


Arifianti, L., Oktarina, R. D., & Kusumawati, I. (2014). Pengaruh Jenis Pelarut Pengektraksi Terhadap Kadar Sinensetin Dalam Ekstrak Daun Orthosiphon stamineus Benth. E-Journal Planta Husada, 2(1), 3–6.

Badar, A. A. (2006). Karakterisasi Sifat Fisiko Kimia dan Mekanik Daun Patat Daun (Phrynium Capitatum) sebagai Bahan Kemasan. Institut Pertanian Bogor.

Damayanti, A., & Fitriana, E. A. (2012). Pemungutan Minyak Atsiri Mawar (Rose Oil) Dengan Metode Maserasi. Jurnal Bahan Alam Terbarukan, 1(2), 1–8. https://doi.org/10.15294/jbat.v1i2.2543

Dewick, P. M. (2009). Medicinal Natural Products: A Biosynthetic Approach: Third Edition. In Medicinal Natural Products: A Biosynthetic Approach: Third Edition (Vol. 5). https://doi.org/10.1002/9780470742761

Dogan, A., Otlu, S., Çelebi, özgür, Kiliçle, P. A., Saglam, A. G., Can Dogan, A. N., & Mutlu, N. (2017). An investigation of antibacterial effects of steroids. Turkish Journal of Veterinary and Animal Sciences, 41(2), 302–305. https://doi.org/10.3906/vet-1510-24

Farooq, M. U., Mumtaz, M. W., Mukhtar, H., Rashid, U., Akhtar, M. T., Raza, S. A., & Nadeem, M. (2020). UHPLC-QTOF-MS/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-ethanolic leaf extract of Butea monosperma. Scientific Reports, 10(1), 1–14. https://doi.org/10.1038/s41598-020-60076-5

Fathoni, A., Saepudin, E., Cahyana, A. H., Rahayu, D. U. C., & Haib, J. (2017). Identification of nonvolatile compounds in clove (Syzygium aromaticum) from Manado. AIP Conference Proceedings, 1862(July). https://doi.org/10.1063/1.4991183

Fathoni, Ahmad, Rudiana, T., & Adawiah, A. (2019). Characterization and antioxidant assay of yellow frangipani flower (Plumeria alba) extract. Jurnal Pendidikan Kimia, 11(1), 1–7. https://doi.org/10.24114/jpkim.v11i1.13034

Fukuda, T., Okazaki, K., & Shinano, T. (2013). Aroma characteristic and volatile profiling of carrot varieties and quantitative role of terpenoid compounds for carrot sensory attributes. Journal of Food Science, 78(11), 1800–1806. https://doi.org/10.1111/1750-3841.12292

Guimarães, A. C., Meireles, L. M., Lemos, M. F., Guimarães, M. C. C., Endringer, D. C., Fronza, M., & Scherer, R. (2019). Antibacterial activity of terpenes and terpenoids present in essential oils. Molecules, 24(13), 1–12. https://doi.org/10.3390/molecules24132471

Harborne, J. B. (1996). Metode Fitokimia Penuntun Cara Modern Menganalisa Tumbuhan. Bandung: Institut Teknologi Bandung.

Jalli, N., Santhi Sri, K. V., Hnamte, S., Pattnaik, S., Paramanantham, P., & Siddhardha, B. (2019). Antioxidant, anti-quorum sensing and anti-biofilm potential of ethanolic leaf extract of Phrynium capitatum and Dryptes indica. Asian Pacific Journal of Tropical Biomedicine, 9(8), 323–332. https://doi.org/10.4103/2221-1691.262082

Lestari, N. S., & Christina. (2018). Doclang, Makanan Tradisional Yang Mulai Tersisihkan. Jurnal Khasanah Ilmu, 9(2), 21–27.

Manyi-Loh, C. E., Clarke, A. M., & Ndip, R. N. (2011). Identification

of volatile compounds in solvent extracts of honeys produced in South Africa. African Journal of Agricultural Research, 6(18), 4327–4334. https://doi.org/10.5897/AJAR11.754

Marković-Housley, Z., Degano, M., Lamba, D., Von Roepenack-Lahaye, E., Clemens, S., Susani, M., … Breiteneder, H. (2003). Crystal structure of a hypoallergenic isoform of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier. Journal of Molecular Biology, 325(1), 123–133. https://doi.org/10.1016/S0022-2836(02)01197-X

Nadaf, M., Nasrabadi, M., & Halimi, M. (2012). GC-MS Analysis of n-Hexane Extract from Aerial Parts of Salvia nemorosa. Middle-East Journal of Scientific Research, 11(8), 1127–1130.

Noviadji, B. R. (2014). Desain Kemasan Tradisional Dalam Konteks Kekinian. Jurnal Fakultas Desain, 1(1), 10–21.

Nurrani, L. (2013). Pemanfaatan tradisional tumbuhan alam berkhasiat obat oleh masyarakat di sekitar cagar alam tangale. Info BPK Manado Volume, 3(1), 1–22.

Perme, N., Choudhury, S. N., Choudhury, R., Natung, T., & De, B. (2015). Medicinal Plants in Traditional Use at Arunachal Pradesh, India. International Journal of Phytopharmacy, 5(5), 86–98. https://doi.org/10.7439/ijpp

Resna, N. (2020). Mencicipi Kuliner Tradisional Doclang yang Kian Menghilang. Retrieved September 6, 2020, from brisik.id website: https://brisik.id/read/53561/mencicipi-kuliner-tradisional-doclang-yang-kian-menghilang

Shang, H. M., Zhou, H. Z., Li, R., Duan, M. Y., Wu, H. X., & Lou, Y. J. (2017). Extraction optimization and influences of drying methods on antioxidant activities of polysaccharide from cup plant (Silphium perfoliatum L.). PLoS ONE, 12(8), 1–18. https://doi.org/10.1371/journal.pone.0183001

Tapfuma, K. I., Mekuto, L., Makatini, M. M., & Mavumengwana, V. (2019). The LC-QTOF-MS/MS analysis data of detected metabolites from the crude extract of Datura stramonium leaves. Data in Brief, 25, 104094. https://doi.org/10.1016/j.dib.2019.104094

Wijaya, D. P., Paendong, J. E., & Abidjulu, J. (2014). Skrining Fitokimia dan Uji Aktivitas Antioksidan dari Daun Nasi (Phrynium capitatum) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil). J Mipa Unsrat Online, 3, 11–15. https://doi.org/10.35799/jm.3.1.2014.3899.




DOI: http://dx.doi.org/10.30870/educhemia.v6i1.9189

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