Devepoment Method Radicula Assay as Fast Method Viability and Vigor for Several Feed Arabica Coffee
Iis Deny Sri Noeryanti, Kartina AM, Fitri Riany Eris
Abstract
Uji pemunculan radikula merupakan salah satu metode untuk mengestimasi vigor benih. Prinsip metode uji pemunculan radikula adalah benih yang berkecambah lambat merupakan tanda awal kemunduran fisiologis benih dan menunjukkan bahwa lot benih tersebut bervigor rendah. Penelitian ini bertujuan untuk memperoleh waktu pengamatan pemunculan radikula yang tepat pada uji pemunculan radikula benih kopi Arabika serta memperoleh korelasi antara hasil uji pemunculan radikula dengan berbagai tolok ukur viabilitas dan vigor benih. Pengujian viabilitas dan vigor benih di laboratorium menggunakan tolok ukur daya berkecambah (DB), rataan waktu perkecambahan (MGT), indeks vigor (IV), kecepatan tumbuh (KCT), bobot kering kecambah normal (BKKN), dan laju pertumbuhan kecambah (LPK). Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok Faktorial yang terdiri dari dua faktor. Faktor pertama adalah varietas benih, terdiri dari tiga taraf yaitu varietas S 795, Gayo 1, dan Sigarar Utang. Faktor kedua adalah periode pengecambahan, terdiri dari lima taraf yaitu 168, 192, 216, 240, dan 264 jam. Hasil penelitian menunjukkan bahwa varietas Sigarar Utang memberikan hasil terbaik terhadap pemunculan radikula sebesar 98.87 %. Periode pengecambahan selama 264 jam memberikan pengaruh terbaik terhadap pemunculan radikula pada benih kopi Arabika. Terdapat interaksi antara varietas dan periode pengecambahan terhadap pemunculan radikula pada benih kopi Arabika (P < 0.05). Hasil uji pemunculan radikula mempunyai korelasi positif yang kuat dengan pengujian viabilitas benih daya berkecambah (r = 0.70), dan berkorelasi positif sangat kuat dengan tolok ukur vigor benih indeks vigor (r = 0.84), kecepatan tumbuh (r = 0.97), berat kering kecambah normal (r = 0.88), dan laju pertumbuhan kecambah (0.80), serta berkorelasi negatif sangat kuat dengan tolok ukur vigor benih rataan waktu perkecambahan (-0.94).
DOI:
http://dx.doi.org/10.33512/jipt.v4i1.16585
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